GWC2 RNA reads - SRA

SRX957568: GWC2 RNA reads
1 ABI_SOLID (AB 5500xl Genetic Analyzer) run: 77.2M spots, 3.2G bases, 2Gb downloads

Design: RNA was extracted from the 0.2 mm filters using the Invitrogen TRIzol® reagent, followed by genomic DNA removal and cleaning using the Qiagen RNase-Free DNase Set kit and the Qiagen Mini RNeasyTM kit. An Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA) was used to assess the integrity of the RNA samples. The Applied Biosystems SOLiD(TM) Total RNA-Seq kit was used to generate the cDNA template library. The SOLiD(TM) EZ Bead system (Life Technologies, Grand Island, NY) was used to perform emulsion clonal bead amplification to generate bead templates for SOLiD(TM) platform sequencing. Samples were sequenced at Pacific Northwest National Laboratory on the 5500XL SOLiD(TM) platform. The 50 bp single reads were trimmed using Sickle with default parameters.

Submitted by: UNIVERSITY OF CALIFORNIA, BERKELEY

Study: Rifle, CO metagenome from aquifer

PRJNA268031 • SRP050083 • All experiments • All runs

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The study encompasses 12 microbial samples sourced from an aquifer adjacent to the Colorado River near the town of Rifle, CO, USA. Samples were taken at 6 different times prior to and during an acetate amendment experiment. For each sample, cells were collected on serial 0.2 and 0.1 µm filters resulting in a total of 12 different samples.

Sample: time point 3 / C; 0.2 filter

SAMN03202999 • SRS749186 • All experiments • All runs

Organism: groundwater metagenome

Library:

Name: 5500XL SOLiD(TM) platform

Instrument: AB 5500xl Genetic Analyzer

Strategy: RNA-Seq

Source: METATRANSCRIPTOMIC

Selection: RANDOM

Layout: SINGLE

Spot descriptor:

1 forward

Runs: 1 run, 77.2M spots, 3.2G bases, 2Gb

Run	# of Spots	# of Bases	Size	Published
SRR1916833	77,195,756	3.2G	2Gb	2015-03-18

ID:: 1359091

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